Journal: The Journal of Biological Chemistry
Article Title: Interleukin-1β Processing Is Dependent on a Calcium-mediated Interaction with Calmodulin *
doi: 10.1074/jbc.M115.680694
Figure Lengend Snippet: Identification of pro-IL-1β interacting proteins on a human proteome microarray. A human proteome microarray was incubated with human recombinant pro-IL-1β (+) or with reagent diluent alone as a negative control (−) and probed with a mouse anti-human IL-1β antibody. An SNR value was generated for each probe set, with an SNR of >3 considered to be a hit. A comprehensive list of all identified pro-IL-1β interacting proteins (hits) is shown, including one representative negative result (for brain protein 44-like) and a positive control (anti-biotin mouse monoclonal IgG). The identity of each protein, the corresponding images for each duplicate set of proteins on the array, and the mean SNR for the duplicate protein spots are shown.
Article Snippet: Two HuProt human protein microarray slides (v.2.0) containing 19,951 probe sets spotted in duplicate were purchased from CDI Laboratories (Mayaguez, PR).
Techniques: Microarray, Incubation, Recombinant, Negative Control, Generated, Positive Control